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KMID : 1161520010050030237
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Animal Cells and Systems
2001 Volume.5 No. 3 p.237 ~ p.241
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Analysis of the short tandem repeat Ioci for STRX 1, HPRTB, ARA, DYS390, DYS392 and DYS393 in Koreans
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Seol Hye-Won
Tun Zaw
Honda Katsuya
Msawa Shogo
Park Kyung-Sook
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Abstract
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Three STR loci (STRX 1[AGAT]n, HPRTB[AGAT]n and ARA[AGC]n) on X chromosome and three other STR loci (D YS390[CTG(A)T]n, DYS392[ATT]n and DYS393[GATA]n) on Y chromosome were analyzed in 154 unrelated healthy Korean subjects. Four loci (STRX1, HPRTB, DYS390 and DYS393) were amplified by quadruplex polymerase chain reaction (PCR) using fluorescent labeled primers (FLP). ARA and DYS392 were amplified separately using single PCR, similarly by using FLP. They were then run in an automated DNA sequencer and were analyzed with Genescan software. We found 7 alleles (308?332 bp) in STRX1, 7 alleles (275?299 bp) in HPRTB, 16 alleles (252?315 bp) in ARA, 6 alleles (203?223 bp) in DYS390, 7 alleles (245?263bp) in DYS392 and 5 alleles (116?132 bp) in DYS393. The *13 (34%), * 13 (51%), *23 (18%), *23 (50%), * 14 (39%) and * 13 (40%) alleles were observed to be the highest frequencies of STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393, respectively. The detection of STR loci on sex chromosomes by quadruplex PCR allows simple determination of sex and identification of an individual.
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KEYWORD
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Quadruplex PCR, X S TRs, Y S TRs, Y Haplotype, Koreans
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